CIRBP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CIRBP antibody was raised against an 11 amino acid synthetic peptide near the internal region of CIRBP (aa 81-91).
ELISA: Antibody detection limit dilution 1:16000 Western Blot: Approximately 18 kDa band observed in mouse and rat testis lysates (calculated MW of 18.6 kDa according to mouse NP_031731.1 and rat NP_112409.2). Recommended concentration: 0.05-0.2 μg/mL. an additional band of unknown identity was also consistently observed at 23 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
CIRBP
(Cold Inducible RNA Binding Protein (CIRBP))