This antibody was raised against the C-terminus of NP_055210.1. However, NCBI has changed the version to NP_055210.2 thus losing the C-terminal epitope.
Purification
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
DAPP1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of DAPP1.
Verified
DAPP1
Reactivity: Human
WB, ELISA
Host: Goat
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 26 kDa band in human heart lysates after 1 μg/mL antibody staining.0 kDa according to AAF14578.1. The 26 kDa band was successfully blocked by incubation with the immunizing peptide. Approximately 30 kDa band observed in human kidney lysates (calculated MW of 32.0 kDa according to AAF14578.1). Recommended concentration: 0.5-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
DAPP1
(Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))