Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize both reported isoforms, as represented by NP_005722 and NP_690601.
Purification
ARPC2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ARPC2 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of ARPC2.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 33-34 kDa band observed in Hela and human skeletal muscle lysates (predicted MW of 34 kDa according to NP_005722). Recommended for use at 0.1-0.5 μg/mL. Immunohistochemistry: In paraffin embedded human spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
ARPC2
(Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))