Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
This antibody will recognize both human FOXG1A and FOXG1B, which are almost completely identical.
Purification
FOXG1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
FOXG1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of FOXG1.
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 48 kDa band observed in human brain lysates (calculated MW of 52.4 kDa according to NP_005240.3 (FOXG1B) and 50.5 kDa according to CAA55038.1 (FOXG1A). Recommended concentration: 0.3-1.0 μg/mL. an additional band of unknown identity was also consistently observed at 70 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.