Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Purification
p70 S6 Kinase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
p70 S6 Kinase antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of p70 S6 Kinase.
ELISA: Antibody detection limit dilution >1:128,000. Western Blot: Approximately 65-70 kDa band observed in human muscle and placenta lysates (predicted MW of 60 kDa according to NP_003152.1). Recommended for use at 0.2-1 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows ribosomal staining in DCT cells. Recommended concentration, 1-2 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
RPS6KB1
(Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))