Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
ACAP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ACAP2 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of ACAP2.
ACAP2
Reactivity: Human
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 350
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 90 kDa band observed in human T cell line MOLT4 lysates (calculated MW of 88.1 kDa according to NP_036419). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
Centaurin beta 2 (ACAP2)
(ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 2 (ACAP2))