ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Specificity
This antibody is expected to recognize both reported isoforms (represented by NP_056057 and NP_631920).
Purification
ARAP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ARAP1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of ARAP1.
ARAP1
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave no signal but low background in human muscle and lung extracts at up to 1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
ARAP1
(ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 1 (ARAP1))