CARD11 antibody (C-Term)

Catalog No. ABIN2465495

Product Details anti-CARD11 Antibody

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CARD11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: CARD11 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CARD11 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of CARD11.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 150+65 kDa bands observed in Jurkat lysate (calculated MW of 133 kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 60 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human spleen shows nuclear staining. Recommended concentration, 2-5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for CARD11

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Alternative Name: CARD11 (CARD11 Products)

Synonyms: BENTA antibody, BIMP3 antibody, CARMA1 antibody, PPBL antibody, 0610008L17Rik antibody, 2410011D02Rik antibody, CARD11 antibody, LOC100232755 antibody, caspase recruitment domain family member 11 antibody, caspase recruitment domain family, member 11 antibody, caspase recruitment domain-containing protein 11 antibody, CARD11 antibody, Card11 antibody, LOC100550812 antibody, card11 antibody

Gene ID: 84433

NCBI Accession: NP_115791

UniProt: Q9BXL7

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, BCR Signaling