HCST antibody (Hematopoietic Cell Signal Transducer) (C-Term)

Details for Product anti-HCST Antibody No. ABIN2465583, Supplier: Log in to see
Antigen
  • HCST
  • DAP10
  • KAP10
  • PIK3AP
  • Dap10
  • hematopoietic cell signal transducer
  • HCST
  • hcst
  • Hcst
Epitope
C-Term
12
2
1
1
1
Reactivity
Human
30
1
1
1
Host
Goat
15
15
Clonality
Polyclonal
Conjugate
This HCST antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
15
13
12
3
1
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Immunogen DAP10 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of DAP10.
Specificity This antibody is expected to recognize isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
Purification DAP10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DAP10 (HCST Antibody Abstract)
Gene ID 10870
NCBI Accession NP_055081, NP_001007470
UniProt Q9UBK5
Application Notes ELISA: Antibody detection limit dilution 1:128,000. Approximately 40 kDa band observed in human spleen lysates (calculated MW of 9.5 kDa according to NP_055081.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wu et al, Science. 1999 Jul 30,285 (5428) :730-2., PMID: 10426994). Recommended concentration: 0.2-0.6 μg/mL. an additional band of unknown identity was also consistently observed at 100 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Concentration 500 μg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Hematopoietic Cell Signal Transducer (HCST) (C-Term) antibody (ABIN2465583) anti-Hematopoietic Cell Signal Transducer (HCST) (C-Term) antibody
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