Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize only one of the three reported isoforms (NP_570722.2, isoform a).
Purification
DYX1C1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
DYX1C1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of DYX1C1.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: lysates of COS1 transfected with full length recombinant human DYX1C1 gave bands at approximately 48 kDa after 0.1 μg/mL antibody staining. In addition, a minor band of 24 kDa is detected consistent with observations with N-terminal specific antibodies. The 75 kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this antibody is used in other assays than Western Blot. IHC: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in some of the neuronal cells. Recommended concentration, 5-10μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.