Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
ENPP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ENPP1 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of ENPP1.
ENPP1
Reactivity: Mouse, Rat
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: In transfected HEK293 transiently expressing ENPP1 a band of approximately 100 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 105 kDa according to NP_006199.2. Recommended concentration, 1-3 μg/mL. Immunohistochemistry: In paraffin embedded liver shows membranous staining in many hepatocytes. This antibody has also been successfully used on human pancreas showing membranous staining of acinar cells. Recommended concentration, 1-2 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.