This antibody is expected to recognize isoforms a, c and d (NP_004091.2, NP_742102.1 and NP_742103.1 respectively).
Purification
EYA4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
EYA4 antibody was raised against a 15 amino acid synthetic peptide near the N-Terminus of EYA4.
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 50 kDa band in human and mouse brain lysates and in pituitary mouse cell line NIH 3T3 after 0.1 μg/mL antibody staining.5 kDa, 67.1 kDa and 69.4 kDa according to NP_004091.2, NP_742102.1 and NP_742103.1 respectively. The 50 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.