Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
Purification
GPX3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GPX3 antibody was raised against a 13 amino acid synthetic peptide near the internal region of GPX3.
GPX3
Reactivity: Rat
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 350
Application Notes
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 26 kDa band observed in mouse kidney and liver lysates (calculated MW of 27.7 kDa according to mouse NP_001077398.1 [isoform1], 25.4 kDa according to mouse NP_032187.2 [isoform 2] and 25.5 kDa according to human NP_002075.2). Recommended concentration: 1-3 μg/mL. IHC: Frozen sections of Porcin Kidney shows vesicular staining of the cytoplasm in PCT epithelial cells. Recommended concentration, 20μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.