Integrin beta 5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Integrin beta 5 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of Integrin beta 5.
ITGB5
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 110 kDa band observed in human ovary and mouse eye lysates (calculated MW of 88.1 kDa according to NP_002204.2). Recommended concentration: 0.1-0.3 μg/mL. Customer feedback: observed 110 kDa in mouse eye was confirmed and not present in knock-out mouse.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.