Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize both reported isoforms (NP_006208.1, NP_001012303.1).
Purification
MEPI antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MEPI antibody was raised against a 14 amino acid synthetic peptide near the internal region of MEPI.
SERPINI2
Reactivity: Human, Mouse, Rat
WB, IF (p), IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 40 kDa band observed in human pancreas lysates (calculated MW of 46.1 kDa according to NP_006208.1 and 47.4 kDa according to NP_001012303.1). Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
SERPINI2
(serpin Peptidase Inhibitor, Clade I (Pancpin), Member 2 (SERPINI2))