Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Specificity
Human Y2R could not be detected in WB by this antibody. Pease note this antibody was designed using the Mouse sequence NP_032757.1, which differs by 1 amino acid from the Human sequence NP_000901.1.
Purification
Npy2r antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Npy2r antibody was raised against a 12 amino acid synthetic peptide near the internal region of Npy2r.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 40+45 kDa bands observed in mouse brain and rat brain lysates (calculated MW of 42.6 kDa according to NP032757.1). Recommended concentration: 0.1-0.3 μg/mL. Immunoprecipitation: A customer successfully used this antibody for IP of human Y2R.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.