PHLDA3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PHLDA3 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of PHLDA3.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 70 kDa band in human placenta lysate after 1 μg/mL antibody staining. This band was successfully blocked by the immunizing peptide. In transfected HEK293 transiently expressing full-length human PHLDA3 (myc and DYKDDDDK tagged), a band of approximately 18 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
PHLDA3
(Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3))
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