This antibody is expected to recognize isoform 1 (NP_006805.1 and NP_848693.1) only, not isoform 2 (NP_848694.1).
Purification
PI31 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PI31 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of PI31.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 35 kDa band observed in human kidney lysates (calculated MW of 29.8 kDa according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approximately 35 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.