PIR51 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PIR51 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of PIR51.
RAD51AP1
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 25 kDa band in human testis lysates at 0.2 μg/mL. This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration, 4-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.