PLA2G1B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PLA2G1B antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of PLA2G1B.
PLA2G1B
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 17-18 kDa band observed in human pancreas lysates (calculated MW of 16.4 kDa according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approximately 18 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.