PRAM1 antibody (C-Term)

Catalog No. ABIN2466137

Product Details anti-PRAM1 Antibody

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRAM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: PRAM1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: PRAM1 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of PRAM1.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 90-100 kDa band seen in Jurkat cell lysates. Recommended for use at 1-3 μg/mL. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79 kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001, 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for PRAM1

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Alternative Name: PRAM1 (PRAM1 Products)

Synonyms: PRAM1 antibody, AY665714 antibody, PML-RAR antibody, PRAM-1 antibody, RGD1564330 antibody, PML-RARA regulated adaptor molecule 1 antibody, PML-RAR alpha-regulated adaptor molecule 1 antibody, PRAM1 antibody, Pram1 antibody

Gene ID: 84106

NCBI Accession: NP_115528

UniProt: Q96QH2

Pathways: Regulation of Leukocyte Mediated Immunity