This antibody is expected to recognize both reported isoforms (NP_892030.3, NP_542783.2).
Purification
RNF36 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
RNF36 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of RNF36.
TRIM69
Reactivity: Human
WB
Host: Rabbit
Polyclonal
RB13944
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 20-25 kDa band in human testis lysates after 1 μg/mL antibody staining. Approximately 55 kDa band observed in mouse testis lysates (calculated MW of 57.3 kDa according to mouse NP_536771.2). Recommended concentration: 0.5-2 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows speckled staining in nuclei of spermatocytes and spermitids. Recommended concentration, 2.5-3.8 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.