SBP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SBP2 antibody was raised against a 13 amino acid synthetic peptide near the internal region of SBP2.
SECISBP2
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 120 kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 Jul,26 (13) :4895-910, PMID: 16782878). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.