Semaphorin 3E antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Semaphorin 3E antibody was raised against a 14 amino acid synthetic peptide near the internal region of Semaphorin 3E.
SEMA3E
Reactivity: Human, Mouse
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 555
Application Notes
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 35 kDa band in human prostate lysates after 0.03 μg/mL antibody staining.2 kDa according to NP_036563.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
SEMA3E
(Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))