SF3B3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SF3B3 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of SF3B3.
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 150 kDa band observed in nuclear HeLa lysate (calculated MW of 136 kDa according to NP_036558.3). Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.