SIPA1 antibody (C-Term)

Catalog No. ABIN2466269

Product Details anti-SIPA1 Antibody

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIPA1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: Both variants represent identical protein (NP_694985.28 and NP_006738.2).

Purification: SIPA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: SIPA1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of SIPA1.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32,000. Approximately 150 kDa band observed in human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112 kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31,272 (44) :28081-8, PMID: 9346962). Recommended concentration: 1-3 μg/mL. an additional band of 55-60 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this antibody is used for other assays than Western Blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for SIPA1

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Alternative Name: SIPA1 (SIPA1 Products)

Synonyms: Spa1 antibody, SPA1 antibody, signal-induced proliferation-associated 1 antibody, Signal peptidase I (leader peptidase I) antibody, signal-induced proliferation associated gene 1 antibody, SIPA1 antibody, sipA1 antibody, Sipa1 antibody

Gene ID: 6494

NCBI Accession: NP_006738

UniProt: Q96FS4

Pathways: Response to Water Deprivation