Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize both reported isoforms (as represented by NP_060190.2 and NP_001013863.1).
Purification
STAP-2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
STAP-2 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of STAP-2.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 48 kDa band observed in human heart lysates (calculated MW of 49.6 kDa according to NP_060190.2). Recommended concentration: 0.05-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cortex shows textured cytoplasm staining in neuronal bodies and processes. Recommended concentration, 1-2μg/mL
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
STAP2
(Signal Transducing Adaptor Family Member 2 (STAP2))