This antibody is expected to recognize both reported isoforms (NP_071320.1 and NP_071505.1).
Purification
TIA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TIA1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of TIA1.
TIA1
Reactivity: Human
WB, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 40 kDa band observed in Jurkat lysate (calculated MW of 41.8 kDa according to NP_071320.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band at 80 kDa and a very strong 26 kDa is also observed. These bands were successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
TIA1
(TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))