This antibody is expected to recognize both reported isoforms, as represented by NP_003843.3 and NP_056989.2.
Purification
TIF1 alpha antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TIF1 alpha antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of TIF1 alpha.
TRIM24
Reactivity: Human, Mouse, Cow, Rat, Horse, Guinea Pig, Rabbit, Pig, Monkey, Bat
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 120 kDa band in human kidney and heart lysates at 1 μg/mL, however prominent bands were also seen at 25 kDa and 45 kDa . Immunohistochemistry: In paraffin embedded human breast carcinoma shows nuclear staining in the distorted lobules. Recommended concentration, 4-6μg/mL
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.