This antibody is expected to recognize an epitope corresponding to aa 396-409 of human TIM50.
Purification
TIM-50 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TIM-50 antibody was raised against a 15 amino acid synthetic peptide near the internal region of TIM-50.
TIMM50
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: experiments gave bands at approximately 40 kDa in human heart lysate after 0.5 μg/mL antibody staining. This band corresponds to earlier findings in literature with different antibodies (PMID: 15044455). This protein has a calculated MW of 50.5 kDa according to NP_001001563. Recommended concentration: 0.5-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
TIMM50
(Translocase of Inner Mitochondrial Membrane 50 Homolog (TIMM50))