Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Purification
WNT3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
WNT3 antibody was raised against a 15 amino acid synthetic peptide near the internal region of WNT3.
WNT3
Reactivity: Human, Mouse
WB, IHC
Host: Rabbit
Polyclonal
PE
Application Notes
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 38 kDa band observed in human brain lysates (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human breast shows strong fibrous staining in myoepithelial cells. Recommended concentration, 3-5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
WNT3
(Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))