ZDHHC8 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ZDHHC8 antibody was raised against a 13 amino acid synthetic peptide near the internal region of ZDHHC8.
ZDHHC8
Reactivity: Mouse
IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 488
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 80-85 kDa band observed in human frontal cortex lysates (calculated MW of 81.4 kDa according to NP_037505.1). Recommended concentration: 1-3 μg/mL. an additional band of 48 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western Blot.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.