APOBEC2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
APOBEC2 antibody was raised against a 15 amino acid synthetic peptide near the internal region (near the N-Terminus) of APOBEC2.
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 26 kDa band observed in human heart and muscle lysates (calculated MW of 25.7 kDa according to NP_006780.1). Recommended concentration: 1-3 μg/mL. an additional band of 90 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
APOBEC2
(Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 2 (APOBEC2))