Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
This antibody is expected to recognize all three reported isoforms (NP_001020366.1, NP_001020365.1, NP_001257.4).
Purification
CES1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CES1 antibody was raised against a 12 amino acid synthetic peptide near the internal region (near C-Terminus) of CES1.
CES1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 55-60 kDa band observed in human liver and Ileum lysates (calculated MW of 62.6 kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approximately 65 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/mL. Immunohistochemistry: In paraffin embedded human lung shows staining of lymphocytes in the vasculature. Recommended concentration, 2-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.