Interleukin 6 antibody (IL6)

Details for Product anti-IL6 Antibody No. ABIN2469708, Supplier: Log in to see
Antigen
  • Il-6
  • ILg6
  • Ifnb2
  • BSF2
  • HGF
  • HSF
  • IFNB2
  • IL-6
  • CHIL-6
  • il6
  • interleukin 6
  • interleukin 6 (interferon, beta 2)
  • interleukin-6
  • Il6
  • IL6
  • il-6
Alternatives
anti-Human Interleukin 6 antibody for Blocking Antibody
Reactivity
Human
576
239
112
37
29
26
21
10
7
7
7
6
4
3
3
2
1
Host
Mouse
382
295
202
38
7
7
5
Clonality (Clone)
Monoclonal ()
Conjugate
This Interleukin 6 antibody is un-conjugated
106
40
26
23
15
13
13
11
11
9
9
9
7
7
6
5
5
5
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
586
534
224
152
123
97
69
65
59
48
41
38
19
17
15
13
12
8
5
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Options
Supplier
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Available images

'Independent Validation' Badge
  • Successfully validated (Immunohistochemistry)
  • by  HIK Hannover
  • No. #100788
  • Date 05/31/2017
  •  
Antigen IL6
Lot Number 19346-1701
Method validated Immunohistochemistry
Positive Control Formalin fixed and plastic embedded human liver and tonsil sections
Negative Control No primary antibody
Notes Passed. The IL6 antibody ABIN2469708 specifically labels the targeted antigen in human liver and tonsil tissue sections in IHC. No signal was detected in no primary antibody negative controls.
Primary Antibody ABIN2469708
Secondary Antibody Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608)
Protocol
  • Disect tissue and fix in 4% buffered formalin for >24h at RT.
  • Plastification in Technovit 9100 plastic embedding system (Heraeus Kulzer) according to manufactuer’s recommencations.
  • Sectioning into 5µm sections on a rotary microtome (Reichert Jung, 1140/Autocut).
  • Deplastify and rehydrate tissue through graded xylene and graded alcohol series:
    • xylene for 20min.
    • MEA (2-Methoxyethyl)acetat zur Synthese (Merck, 8.06061.1000) 2x 20min.
    • 100% ethanol for 1min.
    • 95% ethanol for 1min.
    • 70% ethanol for 1min.
    • H2O for 2min.
    • distilled H2O 2min.
  • Heat induced epitope retrieval in Antigen Retrieval Buffer 4 pH9 (Medac Diagnostika, PMB4-125, lot 161109AB) ON at 60°C in an oven (Memmert, UNB 200).
  • Quench sections in in ice cold distilled H2O 2min.
  • Adjust sections with wash buffer (Medac Diagnostika, B1-30A, lot no161102001) 5min at RT.
  • Incubate sections with primary Interleukin 6 antibody (IL6) (antibodies-online, ABIN2469708, lot 19346-1701) diluted 1:200 in dilution buffere (Medac Diagnostika, B1-31C, lot no 161205001) for 30min at RT. Include no primary antibody negative controls.
  • Rinse sections 3x 5min with wash buffer. Keep negative controls in a separate container.
  • Block endogenous peroxidase with Peroxide Block RTU (Medac Diagnostika, 925B-09, lot no 161103001) for 10min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608) for 30min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Bright DAB (Medac Diagnostika, BS04-110,lot no 160523) for 10min at RT.
  • Rinse sections in distilled H2O.
  • Counterstain with Mayers Haematoxylin (Leica, 3801582E, lot no 3801582E) 5min at RT.
  • Dehydrate in fresh ethanol:
    • 95% ethanol twice for 2min.
    • 99.5% ethanol twice for 2min.
    • Xylene twice for 2min.
  • Mount sections with Celltexx mounting medium (Engelbrecht, 19360, lot no 1716047).
  • Dry sections in a ventilated place.
  • Image acquisition on a Leica Microsystems DM 1000 light microscope with a DFC 320 digital camera. Magnification tonsil sections 40x, liver sections 10x and 20x.
Experimental Notes The immunostaining pattern of human liver and tonsil with ABIN2469708 is consistent with published data.
Validation Images
Immunohistochemistry validation image for anti-Interleukin 6 (IL6) antibody (ABIN2469708) Immunostaining of IL6 in human liver liver (A) and tonsil sections (C) using ABIN2469...
Immunogen IL - 6 monoclonal antibody was raised against Ni-NTA purified recombinant human IL-6 expressed in E. Coli strain BL21 (DE3)
Clone 6D9A1-6C12B1
Isotype IgG1
Purification Ascites
Alternative Name IL-6 (IL6 Antibody Abstract)
Gene ID 3569
UniProt P05231
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer, Receptors
Pathways Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis
Application Notes IL-6 antibody can be used in ELISA starting at 1/200 - 1/800, and immunohistochemistry starting at 10 mg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid, 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice As with all antibodies avoid freeze/thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store IL-6 antibody at 4 °C or -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Interleukin 6 antibody (IL6) (ABIN2469708) anti-Interleukin 6 (IL6) antibody