BrdU antibody (Bromodeoxyuridine)

Details for Product anti-BrDU Antibody No. ABIN2477713, Supplier: Log in to see
Antigen
Reactivity
Chemical
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Host
Mouse
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20
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Clonality (Clone)
Monoclonal ()
Conjugate
This BrdU antibody is un-conjugated
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5
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Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Options
Supplier
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Immunogen Bromodeoxyuridine conjugated to BSA
Clone Bu20a
Isotype IgG1
Characteristics Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Alternative Name BrdU
Target Type Chemical
Background Synonyms: 5-BROMODEOXYURIDINE
Application Notes Flow: 1/25-1/100, ICC 1/25-1/100
For Flow Cytometry use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline, 0.09%Sodium Azide (NaN3)
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended.
Expiry Date 18 months
Supplier Images
Flow Cytometry (FACS) image for anti-Bromodeoxyuridine (BrDU) antibody (ABIN2477713) anti-Bromodeoxyuridine (BrDU) antibody
Product cited in: Innis, Dai, Wu, Buchan, Jacobson: "Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 6, pp. G1376-85, 2010 (PubMed).

Background publications Dyson: "Lameness due to pain associated with the distal interphalangeal joint: 45 cases." in: Equine veterinary journal, Vol. 23, Issue 2, pp. 128-35, 1991 (PubMed).

Magaud, Sargent, Clarke, Ffrench, Rimokh, Mason: "Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 37, Issue 10, pp. 1517-27, 1989 (PubMed).

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