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Calnexin antibody (C-Term) (Atto 488)

CANX Reactivity: Dog WB, IHC, IF, IP, FACS, ICC Host: Rabbit Polyclonal Atto 488
Catalog No. ABIN2481631
  • Target See all Calnexin (CANX) Antibodies
    Calnexin (CANX)
    Binding Specificity
    • 48
    • 17
    • 14
    • 6
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 117
    • 73
    • 72
    • 47
    • 34
    • 31
    • 31
    • 29
    • 29
    • 26
    • 25
    • 25
    • 22
    • 21
    • 20
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dog
    Host
    • 116
    • 23
    • 7
    Rabbit
    Clonality
    • 113
    • 34
    Polyclonal
    Conjugate
    • 71
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Calnexin antibody is conjugated to Atto 488
    Application
    • 122
    • 78
    • 56
    • 55
    • 38
    • 27
    • 27
    • 26
    • 21
    • 14
    • 13
    • 9
    • 8
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Specificity
    Detects the C-terminal domain of Calnexin ~90 kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.
    Cross-Reactivity
    Chicken, Cow, Dog, Drosophila melanogaster, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Quail, Rabbit, Rat, Sheep, Xenopus laevis
    Purification
    Protein A Purified
    Immunogen
    Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
    Top Product
    Discover our top product CANX Primary Antibody
  • Application Notes
    • WB (1:2000)
    • ICC/IF (1:100)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1:2000 dilution of ABIN2481631 was sufficient for detection of Calnexin in 10 μg of HeLa cell lysate by ECL immunoblot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    Calnexin (CANX)
    Alternative Name
    Calnexin (CANX Products)
    Synonyms
    CNX antibody, IP90 antibody, P90 antibody, 1110069N15Rik antibody, AI988026 antibody, Cnx antibody, D11Ertd153e antibody, canx antibody, clgn antibody, clnx antibody, cnx antibody, PP90 antibody, zgc:63524 antibody, wu:fe06b12 antibody, CANX antibody, calnexin antibody, CalNeXin antibody, calnexin L homeolog antibody, CANX antibody, Canx antibody, cnx-1 antibody, canx.L antibody, canx antibody, LOC100283947 antibody
    Background
    Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
    Gene ID
    403908
    NCBI Accession
    NP_001003232
    UniProt
    P24643
    Pathways
    MAPK Signaling, Thyroid Hormone Synthesis
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