TRAP1 antibody (AA 1-59) (APC)

Catalog No. ABIN2482019

Product Details anti-TRAP1 Antibody (APC)

Target: TRAP1 (TNF Receptor-Associated Protein 1 (TRAP1))

Binding Specificity: AA 1-59

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAP1 antibody is conjugated to APC

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~75 kDa, multiple isoforms.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Human recombinant TRAP1 (amino acids 1-59 removed - transit peptide)

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:120)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482019 was sufficient for detection of Trap-1 in 20 μg of Hela Cell lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for TRAP1

Target: TRAP1 (TNF Receptor-Associated Protein 1 (TRAP1))

Alternative Name: TRAP1 (TRAP1 Products)

Synonyms: HSP 75 antibody, HSP75 antibody, HSP90L antibody, TRAP-1 antibody, 2410002K23Rik antibody, fc85a11 antibody, wu:fc85a11 antibody, TNF receptor associated protein 1 antibody, TNF receptor-associated protein 1 antibody, TRAP1 antibody, Trap1 antibody, trap1 antibody

Background: The 90 kDa heat shock protein (HSP90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to beassociated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).

Gene ID: 10131

NCBI Accession: NP_057376

UniProt: Q12931