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SOD3 antibody (AA 227-236) (Alkaline Phosphatase (AP))

SOD3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal Alkaline Phosphatase (AP)
Catalog No. ABIN2482052
  • Target See all SOD3 Antibodies
    SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))
    Binding Specificity
    • 11
    • 7
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 227-236
    Reactivity
    • 66
    • 27
    • 15
    • 2
    Human
    Host
    • 62
    • 19
    • 2
    • 1
    • 1
    • 1
    Rabbit
    Clonality
    • 68
    • 18
    Polyclonal
    Conjugate
    • 42
    • 12
    • 7
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SOD3 antibody is conjugated to Alkaline Phosphatase (AP)
    Application
    • 66
    • 37
    • 34
    • 31
    • 24
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Detects extracellular SOD ~35 kDa.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Peptide Affinity Purified
    Immunogen
    Peptide corresponding to AA 227-236 of human EC SOD
    Top Product
    Discover our top product SOD3 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2482052 was sufficient for detection of ECSOD in 20 μg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))
    Alternative Name
    SOD3 (SOD3 Products)
    Synonyms
    EC-SOD antibody, ECSOD antibody, SOD3 antibody, AI314465 antibody, LOC780439 antibody, fb05f10 antibody, si:dkey-117m1.6 antibody, sod3 antibody, wu:fb05f10 antibody, ECSODPT antibody, CuZnSOD antibody, SODA.4 antibody, superoxide dismutase 3 antibody, superoxide dismutase 3, extracellular antibody, superoxide dismutase 3, extracellular b antibody, superoxide dismutase 1 antibody, superoxide dismutase precursor ,putative antibody, SOD3 antibody, Sod3 antibody, sod3b antibody, sod3 antibody, Sod1 antibody, Smp_095980 antibody
    Background
    Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
    Gene ID
    6649
    UniProt
    P08294
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