HA2 antibody (FITC)

Catalog No. ABIN2482212

Product Details anti-HA2 Antibody (FITC)

Target: HA2 (H(+)-ATPase 2 (HA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HA2 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Cross reacts with Aha1.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Aha2

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:80)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482212 was sufficient for detection of Aha2 in 20 μg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for HA2

Target: HA2 (H(+)-ATPase 2 (HA2))

Alternative Name: AHA2 (HA2 Products)

Synonyms: AHA2 antibody, F9N11.40 antibody, F9N11_40 antibody, H(+)-ATPase 2 antibody, P-TYPE H(+)-ATPASE ISOFORM 2 antibody, PLASMA MEMBRANE PROTON ATPASE 2 antibody, PMA2 antibody, H[+]-ATPase 2 antibody, HA2 antibody

Background: The Arabidopsis protein RIN4 is a well known regulator of plant immunity. The plasma membrane H+-ATPases Aha1 and Aha2 are one class of RIN4-associated proteins (1-5). Aha1 and Aha2 play a crucial role in resisting pathogen invasion- plants use RIN4 to regulate H+-ATPase activity during immune responses, thereby controlling stomatal apertures during pathogen attack (1). Wild type AHA2 has been found to be localized to the plasma membrane, and has also been found in the ER (4).

Gene ID: 130872

Pathways: Cellular Glucan Metabolic Process, Proton Transport