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BCAN antibody (AA 219-655) (HRP)

BCAN Reactivity: Rat WB, ICC, IF Host: Mouse Monoclonal S294A-6 HRP
Catalog No. ABIN2483320
  • Target See all BCAN Antibodies
    BCAN (Brevican (BCAN))
    Binding Specificity
    • 13
    • 8
    • 7
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 219-655
    Reactivity
    • 32
    • 18
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 26
    • 20
    • 2
    Mouse
    Clonality
    • 30
    • 18
    Monoclonal
    Conjugate
    • 20
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This BCAN antibody is conjugated to HRP
    Application
    • 38
    • 20
    • 17
    • 16
    • 13
    • 13
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Detects ~140 kDa (and smaller due to proteolytic cleavage).
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 219-655 of rat Brevican
    Clone
    S294A-6
    Isotype
    IgG2b
    Top Product
    Discover our top product BCAN Primary Antibody
  • Application Notes
    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2483320 was sufficient for detection of Brevican in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    BCAN (Brevican (BCAN))
    Alternative Name
    Brevican (BCAN Products)
    Synonyms
    fi32a12 antibody, wu:fi32a12 antibody, BCAN antibody, Xbcan antibody, Cspg7 antibody, ALPBRE antibody, BEHAB antibody, CSPG7 antibody, brevican antibody, brevican L homeolog antibody, bcan antibody, BCAN antibody, bcan.L antibody, Bcan antibody
    Background
    Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.
    Gene ID
    25393
    NCBI Accession
    NP_001028837
    UniProt
    P55068
    Pathways
    Glycosaminoglycan Metabolic Process
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