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CAMKII gamma antibody (Atto 488)

CAMK2G Reactivity: Rat WB, IHC, ELISA, IP, IF, ICC Host: Mouse Monoclonal 22B1 Atto 488
Catalog No. ABIN2484910
  • Target See all CAMKII gamma (CAMK2G) Antibodies
    CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))
    Reactivity
    • 69
    • 61
    • 46
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Rat
    Host
    • 54
    • 32
    • 1
    Mouse
    Clonality
    • 49
    • 38
    Monoclonal
    Conjugate
    • 41
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This CAMKII gamma antibody is conjugated to Atto 488
    Application
    • 58
    • 36
    • 32
    • 26
    • 22
    • 15
    • 11
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Synthetic peptide
    Clone
    22B1
    Isotype
    IgG1
    Top Product
    Discover our top product CAMK2G Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml was sufficient for detection of 0.2 μg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    CAMKII gamma (CAMK2G) (Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G))
    Alternative Name
    CaMKII (CAMK2G Products)
    Synonyms
    CAMK antibody, CAMK-II antibody, CAMKG antibody, 5930429P18Rik antibody, Camkg antibody, calcium/calmodulin dependent protein kinase II gamma antibody, calcium/calmodulin-dependent protein kinase II gamma antibody, CAMK2G antibody, Camk2g antibody
    Background
    CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
    Gene ID
    25400
    NCBI Accession
    NP_037052
    UniProt
    P11275
    Pathways
    WNT Signaling, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity
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