ATP7A antibody (AA 42-61) (HRP)

Catalog No. ABIN2485193

Product Details anti-ATP7A Antibody (HRP)

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Binding Specificity: AA 42-61

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATP7A antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~180 kDa in rat brain membrane preparations.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1

Clone: S60-4

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:500)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2485193 was sufficient for detection of Copper-transporting ATPase1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ATP7A

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Alternative Name: ATP7A (ATP7A Products)

Synonyms: ATP7A antibody, cal antibody, wu:fc43e01 antibody, zgc:153422 antibody, zgc:158633 antibody, DDBDRAFT_0218568 antibody, DDBDRAFT_0235190 antibody, DDB_0218568 antibody, DDB_0235190 antibody, atpase antibody, Atp7a antibody, kal antibody, atp7a antibody, DSMAX antibody, MK antibody, MNK antibody, SMAX3 antibody, Blo antibody, DXHXS1608e antibody, I14 antibody, Mo antibody, blotchy antibody, br antibody, brindled antibody, mottled antibody, Mnk antibody, ATPase copper transporting alpha antibody, ATPase, Cu++ transporting, alpha polypeptide antibody, P-type ATPase antibody, ATP synthase subunit a antibody, copper-transporting ATPase 1 antibody, ATP7A antibody, atp7a antibody, LOC100049514 antibody, Atp7a antibody, LOC412379 antibody

Background: The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-trans locating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage.

Gene ID: 538

NCBI Accession: NP_000043

UniProt: Q04656

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process