PUMA antibody (C-Term) (Atto 594)

Catalog No. ABIN2485947

Product Details anti-PUMA Antibody (Atto 594)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is conjugated to Atto 594

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.

Cross-Reactivity: Chicken, Human, Mouse

Purification: Protein A Purified

Immunogen: C-terminal amino acids of human PUMA

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2485947 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 0.02 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA (BBC3 Products)

Synonyms: BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody

Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Gene ID: 27113

NCBI Accession: NP_001120712

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity