ING1 antibody (N-Term) (Atto 594)

Catalog No. ABIN2486904

Product Details anti-ING1 Antibody (Atto 594)

Target: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ING1 antibody is conjugated to Atto 594

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~33 kDa.

Cross-Reactivity: Human, Mouse

Purification: Peptide Affinity Purified

Immunogen: Mouse p33 ING1 N-terminal peptide -KLH conjugates

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

0.25 mg/ml was sufficient for detection of ABIN2486904 in lysates prepared from human melanoma cell lines by western blot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: TBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ING1

Target: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Alternative Name: ING1 (ING1 Products)

Background: The p33ING1 protein encodes a 33-kD, 294-amino acid protein that displays the characteristics of a tumor suppressor gene. The p33ING1 protein is a regulator of cell cycle, senescence, and apoptosis. It can mediate growth arrest, anchor dependent growth and influence chemo sensitivity (1-4). It also has a proposed role as a growth regulator, which is consistent with its location in the nucleus (5). It has been demonstrated that the ING1 gene has three exons and that four mRNA variants are transcribed from three different promoter regions (p33 ING1, p24 ING1, p27 ING1, p47 IG1) (6, 7). ING1 can possibly influence the above biological functions depending on which variant is expressed (1-4).

Gene ID: 26356

NCBI Accession: NP_036049

UniProt: Q9QXV3

Pathways: Protein targeting to Nucleus, Autophagy