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Antibodies
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anti-Collagen, Type II (COL2) antibody
Collagen, Type II (COL2) antibody
| Antigen |
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| Synonyms |
COL2A1, LOC100009005 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Radioimmunoassay (RIA), Western Blotting (WB), Western Blotting (WB)
ELISA (65), Immunofluorescence (IF) (65), Western Blotting (WB) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (35), Flow Cytometry (FACS) (26), Radioimmunoassay (RIA) (19), Immunohistochemistry (IHC) (10), Immunocytochemistry (ICC) (9), Immunoprecipitation (IP) (9), Dot Blot (Dot) (3), Functional Studies (Func) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN249265 |
| Quantity |
0.1 ml (app. 1 mg/ml) (Variants) |
| Price |
463.13 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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Collagen Type II
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Immunogen
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Purified collagen type II from fetal murine cartilage
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Format
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Purified, Lyophilized
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Affinity purified antibody lyophilized from phosphate buffered solution, no BSA and preservative added!
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Specificity
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Murine collagen type II 100%, murine collagen type I, III and IV <0.1%, Mouse fibronectin < 0.1% Cross-reactivity with bovine collagen type II in ELISA
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Application Notes
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IFA, IHC(P), ELISA, RIA , After de-waxing the tissue slices they are treated with 0.2% hyaluronidase in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Mouse cartilage
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Concentration
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app. 1 mg/ml
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Storage
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2 - 8°C or aliquots at -20°C.
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Restrictions
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For Research Use only
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Alternatives for antigen "Collagen, Type II (COL2)", type "Antibodies"
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Hosts
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Rabbit (69), Mouse (29), Goat (14), Bovine (2)
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Reactivities
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Human (53), Cow (Bovine) (35), Mouse (Murine) (27), Chicken (24), Rat (Rattus) (24), Dog (Canine) (15), Pig (Porcine) (15), Rabbit (15)
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Applications
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ELISA (65), Immunofluorescence (IF) (65), Western Blotting (WB) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (35), Flow Cytometry (FACS) (26), Radioimmunoassay (RIA) (19), Immunohistochemistry (IHC) (10), Immunocytochemistry (ICC) (9), Immunoprecipitation (IP) (9), Dot Blot (Dot) (3), Functional Studies (Func) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (11), None (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Ab-2 (1), Ab-3 (1)
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