Collagen, Type V, alpha 1 (COL5A1) antibody

Antigen: Collagen, Type V, alpha 1 (COL5A1)

Synonyms: AI413331, COL5A1, cha1V

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Radioimmunoassay (RIA)

Catalog no.: ABIN249277

Additional Information

Alternative name: Collagen Type V

Immunogen: Purified collagen type V from human placenta

Format: Purified, Lyophilized

Isotype: IgG  (Matching secondary antibodies)

Description: Affinity purified antibody lyophilized from phosphate buffered solution, no BSA and preservative added!

Specificity: Human collagen type V 100% binding in RIA at 1:100 dilution, cross-reactivity with human collagen type I, III and IV <0.1%, human fibronectin and laminin <0.1% Cross-reactive with cattle and pig

Application Details

Application Notes: IFA, IHC(P), ELISA, RIA , After de-waxing the tissue slices they are treated with 0.2% hyaluronidase in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Human placenta

Concentration: app. 1 mg/ml

Buffer: 0.1 ml IgG-fraction affinity purified, lyophilized from PBS , no BSA and preservative added.

Storage: 2 - 8°C or aliquots at -20°C.

Restrictions: For Research Use only