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25(OH)Vitamin D3-1-alpha-Hydroxylase antibody

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Antigen
Reactivity
Mouse (Murine), Human
1
1
Host
Sheep
1
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
1
1
Supplier
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Immunogen Synthetic peptide from the coding sequence of murine 25(OH)-Vitamin-D3-1-alpha-Hydroxylase (aa 266-289)
Isotype IgG
Specificity 25(OH)Vitamin D3-1-alpha-Hydroxylase, 56 kDa Protein in Western Blot
Purification Ig Fraction
Application Notes The polyclonal antibody is well suited for the detection of 25(Hydroxy)Vitamin D3-1-alpha-Hydroxylase in tissues and tissue extracts e.g. in HKC-8 cell line from human proximal tubules. Working dilution: 1/500 (depending on fixation, species, unmasking method and secondary system)
Restrictions For Research Use only
Format Liquid
Concentration app. 7 mg/ml
Buffer glycine buffered NaCl solution pH 7.4, contains 0.1% sodium azide, 0.1% Epsilon-amino-n-caproic acid, 0.01% Benzamidine and 1 mM EDTA
Preservative Sodium azide, Benzamidine, EACA
Storage 4 °C
Background publications Agic, Xu, Altgassen, Noack, Wolfler, Diedrich, Friedrich, Taylor, Hornung: "Relative expression of 1,25-dihydroxyvitamin D3 receptor, vitamin D 1 alpha-hydroxylase, vitamin D 24-hydroxylase, and vitamin D 25-hydroxylase in endometriosis and gynecologic cancers." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 14, Issue 5, pp. 486-97, 2007

Bland, Markovic, Hills, Hughes, Chan, Squires, Hewison: "Expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in pancreatic islets." in: The Journal of steroid biochemistry and molecular biology, Vol. 89-90, Issue 1-5, pp. 121-5, 2004

Zehnder, Bland, Walker, Bradwell, Howie, Hewison, Stewart: "Expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in the human kidney." in: Journal of the American Society of Nephrology : JASN, Vol. 10, Issue 12, pp. 2465-73, 2000

Bland, Walker, Hughes, Stewart, Hewison: "Constitutive expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in a transformed human proximal tubule cell line: evidence for direct regulation of vitamin D metabolism by calcium." in: Endocrinology, Vol. 140, Issue 5, pp. 2027-34, 1999

Takeyama, Kitanaka, Sato, Kobori, Yanagisawa, Kato: "25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis." in: Science (New York, N.Y.), Vol. 277, Issue 5333, pp. 1827-30, 1997