Staphylococcal Protein A (SPA) antibody

Antigen: Staphylococcal Protein A (SPA)

Clonality: Polyclonal

Host: Chicken

Reactivity: Staphylococcus aureus (S. aureus)

Application: Western Blotting (WB)

Catalog no.: ABIN249347

Additional Information

Alternative name: Staphylococcal Protein A

Immunogen: purified, full length recombinant protein P38507

Format: Liquid

Description: Protein A is a surface protein of S. aureus which binds IgG molecules by their Fcregion. In serum, the bacteria will bind IgG molecules in the wrong orientation ontheir surface, which hinders opsonization and phagocytosis. Mutants of S. aureuslacking protein A are more efficiently phagocytosed in vitro and mutants ininfection models have diminished virulence. Due to its affinity for the Fc region ofmany mammalian immunoglobulins, protein A is considered a universal reagent inbiochemistry and immunology.

Specificity: Predicted reactivity: Staphylococcus aureus.

Application Details

Application Notes: Recommended Dilution 1: 5 000 with standard ECL (WB), 1: 5 000 (ELISA). Additional Information: the IgY fraction is isolated by a two-step PEG precipitation procedure followed byammonium sulphate precipitation .

Buffer: Total IgY fraction at 1 mg/ml in 0.9% NaCl, 0.1% sodiumazide

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Research Area: Immunology

Restrictions: For Research Use only