Auxin Efflux Carrier Pin2 (Pin2) antibody

Antigen: Auxin Efflux Carrier Pin2 (Pin2)

Clonality: Polyclonal

Host: Chicken

Reactivity: Arabidopsis thaliana

Application: Immunocytochemistry (ICC)

Catalog no.: ABIN249348

Additional Information

Immunogen: KLH-conjugated peptide derived from AtPIN2 sequence At5g57090

Format: Lyophilized

Description: Arabidopsis thaliana auxin efflux carrier component AtPIN2 encoded by theAtPIN2 gene (also known as EIR1 and AGR1). AtPIN proteins are asymmetricallylocalized within plant plasma membranes and mediate polar auxin transport.AtPIN2 is a key regulator of the response of Arabidopsis roots to gravity.

Specificity: Predicted reactivity: dicots including: Medicago truncatula, Pisum sativum, Vitis viniferamonocotsincluding: Oryza sativa, Triticum aestivum, Zea mays, Populus tremula x Populustremuloides, moss: Physcomitrella patens.

Application Details

Application Notes: Recommended Dilution 1 : 300 - 1: 600 (ICC). Additional Information: this antibody does not work in western blot application on Arabidopsis thaliana .

Purity: Affinity purified

Buffer: affinity purified IgY PBS pH 8.5 + 0.02% sodium azide lyophilized

Storage: Store lyophilized/reconstituted at -20°C, for reconstitution add glycerol to a final of50% (v/v) and store at -20°C for up to a year. Once reconstituted make aliquots toavoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Men, Boutte, Ikeda et al.: "Sterol-dependent endocytosis mediates post-cytokinetic acquisition of PIN2 auxin efflux carrier polarity." in: Nature cell biology, Vol. 10, Issue 2, pp. 237-44, 2008 (PubMed).